De novo assembly of draft genome and transcriptome of Trypanosoma b. brucei EATRO1125 (AnTaR1 serodeme) strain

Trypanosoma brucei spp, unicellular parasites causing human and animal trypanosomiasis, are transmitted between mammals by tsetse flies. Periodic changes in variant surface glycoproteins (VSG) which form the parasite coat in the mammal, allow them to evade the host immune response. Different isolates of T. brucei show heterogeneity in their repertoires of VSG genes and have single nucleotide polymorphisms that can impact on genome editing. T. b. brucei EATRO1125 (AnTaR1 serodeme) is an isolate that is used increasingly often because it is pleomorphic in mammals and fly transmissible, two characteristics that have been lost by the most commonly used laboratory stocks. We present a draft genome of EATRO1125, including contigs for the intermediate and mini-chromosomes that serve as repositories of VSG genes. In addition, de novo transcriptome assemblies were performed using Illumina sequences from tsetse-derived trypanosomes.

布氏锥虫亚种（Trypanosoma brucei spp.）是引发人类与动物锥虫病的单细胞寄生虫，其传播媒介为采采蝇，可在哺乳动物之间完成传播。构成哺乳动物宿主体内寄生虫外被的变异表面糖蛋白（variant surface glycoproteins, VSG）会发生周期性变化，使寄生虫能够逃避宿主的免疫应答。布氏锥虫的不同分离株在VSG基因库方面存在异质性，且携带可影响基因组编辑过程的单核苷酸多态性。布氏布氏锥虫（Trypanosoma brucei brucei）EATRO1125（AnTaR1血清型）是一种应用愈发广泛的分离株，因其在哺乳动物体内具有多形性且可经采采蝇传播——这两项特性在当前最常用的实验室菌株中均已丢失。本研究公布了EATRO1125的基因组草图，其中包含作为VSG基因储存库的中间染色体与微型染色体的重叠群（contigs）。此外，本研究利用采采蝇来源的锥虫的Illumina测序数据完成了从头转录组组装。