Transcriptome sequencing of Antheraea pernyi

The Antheraea pernyi strain Qing 6 exhibiting yellow-cyan skin color was used for transcriptome sequencing of the cuticle. The larvae were reared on Quercus wutaishanica Mayr in the field. The fifth instar larvae at day 5 were used to collect the epidermis, midgut and hemolymph. The hemolymph was first collected and immediately frozen in liquid nitrogen. The epidermis was dissected on the ice with the removal of the attached muscles and fat bodies. The isolated epidermis and midgut were washed with insect physiological saline, immediately frozen in liquid nitrogen.

本研究以表皮呈黄青色的柞蚕（Antheraea pernyi）青6品系为材料，开展其体壁角质层（cuticle）的转录组测序。实验所用幼虫以五台山栎（Quercus wutaishanica Mayr）为寄主，在野外环境中饲养。选取饲养至第5天的五龄幼虫，采集其表皮（epidermis）、中肠（midgut）与血淋巴（hemolymph）。其中血淋巴采集完成后，立即置于液氮中冷冻保存。表皮样本在冰面上进行解剖操作，剔除附着的肌肉与脂肪体（fat bodies）。分离得到的表皮与中肠经昆虫生理盐水（insect physiological saline）冲洗后，立即置于液氮中冷冻保存。