Open-Field Blast Injury Disrupts Corneal Gene Expression Linked to Ion Transport, Sensory Perception, and Neural Signaling

This study aimed to investigate the transcriptomic alterations in the cornea following open-field blast injury (OFBI) for the first time via RNA sequencing (RNA-Seq) with the objective of identifying key molecular signatures and pathways underlying OFBI-induced corneal pathology. Overall design: A total of 64 C57BL/6J mice (32 OFBI-exposed, 32 naïve controls) were used in the study. OFBI was induced using 350 g of Composition C-4 explosive at a standoff distance of 3 m. RNA was extracted from pooled corneas, and RNA-Seq libraries were prepared and sequenced on an Illumina NovaSeq 6000 platform.

本研究首次通过RNA测序（RNA sequencing, RNA-Seq）探究开阔场爆炸损伤（open-field blast injury, OFBI）后角膜的转录组学改变，旨在识别OFBI诱导角膜病变的关键分子特征与通路。实验设计概述：本研究共使用64只C57BL/6J小鼠，其中32只接受OFBI造模，32只为未处理对照。采用350克C-4炸药（Composition C-4），以3米对峙距离诱导OFBI模型。从混合角膜组织中提取RNA，构建RNA测序文库，并在Illumina NovaSeq 6000测序平台上完成测序。