Bacterial communities in Texcoco alkaline saline soils amended with young maize plants or its hemi(cellulose) fraction

We study three soils of the former lake Texcoco with different electrolytic conductivity (1.9 dS m-1, 17.3 dS m-1 and 33.4 dS m-1) and pH (9.3, 10.4 and 10.3) amended with young maize plants and their neutral detergent fibre (NDF) fraction and incubated aerobically in the laboratory for 14 days while the soil bacterial community structure was monitored by means of 454-pyrosequencing of their 16S rRNA marker gene

本研究选取特斯科科湖旧址（Texcoco）的3份土壤样品开展研究，其电导率（electrolytic conductivity）分别为1.9 dS m⁻¹、17.3 dS m⁻¹与33.4 dS m⁻¹，pH值依次为9.3、10.4及10.3。向上述土壤中添加入年轻玉米植株及其中性洗涤纤维（neutral detergent fibre, NDF）组分后，在实验室环境中开展为期14天的好氧培养；同时借助对16S rRNA标记基因（16S rRNA marker gene）的454焦磷酸测序（454-pyrosequencing）技术，对培养过程中的土壤细菌群落结构进行动态监测。