Oral shedding of striped skunks (Mephitis mephitis) experimentally infected with an avian influenza virus (H4N6).
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aOral shedding was assessed via oral swabs by RRT-PCR. Results are presented as log10 PCR EID50 equivalents/mL. A dash “--” indicates that no viral RNA was detected. The term “nt” indicates the sample was not taken or there was insufficient sample volume to conduct the test.b* = Live virus confirmed by virus isolation in eggs. Only samples from 1–10 DPI were tested.cA single animal was sampled on 16 DPI (nt = sample not taken).dS = Suspect positive: two wells positive but Ct>38.eIndividual was a mock inoculated control skunk housed in the same animal room, but in a separate pen.
a. 口腔排毒量通过口腔拭子样本采用实时逆转录聚合酶链式反应(RRT-PCR)进行检测,结果以log10 PCR测定的50%鸡胚感染量(EID50)当量/mL形式呈现。破折号"--"表示未检测到病毒RNA;术语"nt"表示未采集样本或样本量不足无法开展检测。 b. * 代表经鸡胚病毒分离确认的活病毒,仅对感染后天数(days post inoculation, DPI)1~10的样本进行了检测。 c. 仅1只动物于感染后16日龄进行了采样(nt:未采样)。 d. S = 疑似阳性:2个反应孔呈阳性,但Ct值>38。 e. 该个体为模拟接种的对照臭鼬,饲养于同一动物房,但位于独立笼舍中。




