Remodeling of gene expression during C. elegans hibernation. [Ribo-Seq]. Remodeling of gene expression during C. elegans hibernation. [Ribo-Seq]

In the wild, many animals respond to cold temperatures by entering hibernation. In the clinic, controlled cooling is used widely in transplantation and emergency medicine. Yet, the molecular mechanisms the cells use in response to cold remain largely unexplored. One aspect of cold adaptation is a global downregulation of protein synthesis. Here, we performed total RNA sequencing combined with ribosome profiling to assess specific mRNA levels and their translational status during Caenorhabditis elegans hibernation. Overall design: Total RNA sequencing and ribosome profiling analysis was performed on synchronzied wild-type animals that were grown at different temperatures. For the 20°C condition, animals were grown from the L1 stage to 1-day-old adults at 20°C before collection. For the 10°C condition, animals were grown from the L1 stage to 1-day-old adults at 20°C, after which they were grown for 2 hours at 10°C before collection. For the 4°C 1, 3, or 5 days conditions, animals were grown from the L1 stage to 1-day-old adults at 20°C, after which they were grown for 2 hours at 10°C followed by 1, 3, or 5 days at 4°C before collection.

在自然环境中，多数动物会通过进入冬眠状态来应对低温。而在临床场景中，控制性降温已广泛应用于器官移植与急诊医学领域。然而，细胞应对低温的分子机制在很大程度上仍未得到充分探索。低温适应的一个典型特征是蛋白质合成的全局性下调。本研究中，我们结合总RNA测序（total RNA sequencing）与核糖体谱分析（ribosome profiling），旨在探究秀丽隐杆线虫（Caenorhabditis elegans）冬眠过程中特定mRNA的表达水平及其翻译状态。实验设计：本研究对不同温度下培养的同步化野生型线虫进行总RNA测序与核糖体谱分析。20℃组：将线虫从L1期培养至1日龄成虫后，收集样本。10℃组：先将线虫从L1期培养至1日龄成虫（20℃环境），随后在10℃环境下培养2小时后收集样本。4℃培养1、3或5天组：先将线虫从L1期培养至1日龄成虫（20℃环境），先在10℃环境下培养2小时，再置于4℃环境分别培养1、3或5天后收集样本。