Nucleosome position mapping by micrococcal nuclease analysis of S. cerevisiae cells in raffinose and galactose containing media. Nucleosome position mapping by micrococcal nuclease analysis of S. cerevisiae cells in raffinose and galactose containing media

We analyed the nucleosome positions by using 2 concentrations of micrococcal nuclease of yeast strains that were grown in raffinose and galactose containing media (synthetic complete). Overall design: Mnase-seq of 2 strains grown in raffinose and 2 strains grown in galactose, each at two different concentrations of MNase

我们通过两种浓度的微球菌核酸酶（micrococcal nuclease, MNase），分析了分别在含棉子糖（raffinose）与半乳糖（galactose）的合成完全培养基（synthetic complete media）中培养的酵母菌株的核小体定位情况。实验整体设计：对在棉子糖培养基中培养的2株酵母菌株，以及在半乳糖培养基中培养的2株酵母菌株，均分别使用两种不同浓度的MNase开展微球菌核酸酶测序（MNase-seq）实验。