Untargeted lipidomic profiling of the striatum in G2019S LRRK2 knockin mice under a specialized diet

This Zenodo deposit contains a publicly available description of the Dataset: Title: "Untargeted lipidomic profiling of the striatum in G2019S LRRK2 knockin mice under a specialized diet". Description: G2019S LRRK2 knockin mice and their wild-type littermates were maintained on a normal chow diet (23.7% kcal from fat) for the first 11 months of life. At that time, animals were randomly assigned to either a high-fat diet group (60% kcal from fat) or a control diet group (10% kcal from fat). Mice remained on their respective diets for an additional five months. At 16 months of age, mice were anesthetized with an isoflurane vaporizer, tissue were then collected and immediately snap frozen in liquid nitrogen. Frozen tissues were cryopulverized, homogenized and processed using the Bligh-Dyer method. Lipid identifications were assigned using LipidSearch (v5.0, Thermo Fisher Scientific) which was used to generate a compound list, and lipid peak areas were quantified in Skyline. Each experimental group contained biological replicates (n = 5-6 per group). This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual. This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF). This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset本Zenodo存档包含某公开可用数据集的相关描述： 数据集标题："特殊膳食干预下G2019S型富亮氨酸重复激酶2（LRRK2）敲入小鼠纹状体的非靶向脂质组学分析" G2019S型LRRK2敲入小鼠及其野生型同窝仔鼠在生命前11个月饲喂普通维持饲料（热量占比23.7%来自脂肪）。待小鼠饲养至11月龄时，将其随机分为两组：高脂饲料组（热量占比60%来自脂肪）与对照饲料组（热量占比10%来自脂肪），并继续饲喂5个月。当小鼠达到16月龄时，采用异氟烷蒸发器实施麻醉，采集脑组织后立即置于液氮中速冻。冷冻组织经低温粉碎、匀浆处理后，采用布莱-戴尔（Bligh-Dyer）法进行脂质提取。脂质鉴定通过LipidSearch（v5.0，赛默飞世尔科技）完成，该软件用于生成化合物列表；脂质峰面积则通过Skyline软件进行定量分析。各实验组均设置生物学重复（每组n=5~6）。 本数据集通过ASAP CRN云平台（cloud.parkinsonsroadmap.org）向科研人员开放，访问申请的具体操作指南可查阅用户手册。 本研究由Aligning Science Across Parkinson's协作研究网络（ASAP CRN）通过迈克尔·J·福克斯帕金森病研究基金会（MJFF）资助完成。 本Zenodo存档由ASAP CRN云平台工作人员代表数据集作者创建，可为CRN云平台数据集提供可引用的学术参考。