Antibacterial Activity of a New Ready-To-Use Calcium Silicate-Based Sealer

Abstract The aim of this study was to evaluate the antibacterial potential of a calcium silicate-based sealer (Bio-C Sealer, Angelus) against common bacteria in primary and secondary endodontic infections. Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus mutans were exposed to fresh Bio-C Sealer for 24 h by the agar diffusion method (n=5). Additionally, the antibacterial activity was investigated against E. faecalis and S. mutans biofilms (48 h old) grown in discs with 4 mm in diameter and 2 mm in height. (n=3) of set discs of Bio-C Sealer (Angelus), EndoFill (Dentsply-Mallefer), Sealer 26 (Dentsply), AH Plus (Dentsply), Sealapex (Sybron-Endo) and EndoSequence BC Sealer (Brasseler). The antibacterial activity was evaluated by colony forming unity (CFU) counting using ImageJ software. Data were compared by one-way ANOVA followed by Holm-Sidak test (a=5%). Fresh Bio-C Sealer exhibited antimicrobial activity against all bacteria evaluated by agar diffusion method, except for S. mutans. Set discs of all endodontic sealers tested showed similar CFU values for E. faecalis (p>0.05). S. mutans in biofilms showed higher susceptibility to EndoFill compared with the other sealers (p<0.05). In conclusion, the results indicate that fresh Bio-C Sealer does not inhibit S. mutans growth, but exhibits antibacterial activity against E. faecalis, S. aureus, P. aeruginosa and E. coli. After setting, the Bio-C Sealer exhibits an antimicrobial potential comparable to that of the other sealers evaluated in E. faecalis biofilm, but lower than that of EndoFill for S. mutans biofilm.

摘要 本研究旨在评估硅酸钙基根管封闭剂（Bio-C Sealer，Angelus）对原发性及继发性牙髓感染常见致病菌的抗菌活性。采用琼脂扩散法，将粪肠球菌（Enterococcus faecalis）、大肠埃希菌（Escherichia coli）、铜绿假单胞菌（Pseudomonas aeruginosa）、金黄色葡萄球菌（Staphylococcus aureus）及变形链球菌（Streptococcus mutans）与新鲜Bio-C Sealer共孵育24小时，每组样本量n=5。此外，本研究还针对在直径4mm、高度2mm的圆盘培养基中培养48小时的粪肠球菌及变形链球菌生物膜，考察了该封闭剂的抗菌活性。实验使用固化后的Bio-C Sealer（Angelus）、EndoFill（Dentsply-Mallefer）、Sealer 26（Dentsply）、AH Plus（Dentsply）、Sealapex（Sybron-Endo）及EndoSequence BC Sealer（Brasseler）的圆盘样本，每组样本量n=3。采用ImageJ软件计数菌落形成单位（colony forming unity, CFU）以评估抗菌活性。数据采用单因素方差分析（one-way ANOVA）后辅以霍姆-西达克检验（Holm-Sidak test）进行组间比较，检验水准α=5%。琼脂扩散法检测结果显示，新鲜Bio-C Sealer对除变形链球菌外的所有受试菌株均具有抗菌活性。所有受试根管封闭剂的固化圆盘样本对粪肠球菌生物膜的菌落形成单位值均无显著差异（p>0.05）。与其余受试封闭剂相比，变形链球菌生物膜对EndoFill的敏感性更高（p<0.05）。综上，本研究结果表明，新鲜Bio-C Sealer无法抑制变形链球菌的生长，但对粪肠球菌、金黄色葡萄球菌、铜绿假单胞菌及大肠埃希菌具有抗菌活性。固化后的Bio-C Sealer对粪肠球菌生物膜的抗菌潜力与其余受试封闭剂相当，但对变形链球菌生物膜的抗菌活性低于EndoFill。