Messenger RNA transcript expression in the pig (S. scrofa)

90K Combimatrix gene expression microarrays were composed by 17,048 replicated probes and 963 not replicated specific for the Ensembl (Ver. 56) Transcripts (protein coding + pseudogenes + retrotransposed elements), 11,363 replicated probes specific for the UniGene clusters (Ver. 38) of length comprised between 778 nt and 1,348 nt, and 28,790 single probes specific for the remaining UniGene clusters. These microarrays were used to study gene expression in 14 different pig tissues (Atrium Sinister; Skin; Liver; W_B_A: White Blood Cells from Arteriosum blood; W_B_V: White Blood Cells from Venosum blood; Lymph Node; Tongue; Spleen; Skeletal Muscle; Lung; Kidney; Stomach; Adipose Tissue; Ventricle Sinister). Gene expression was correlated with the micro-RNA expression in the same tissues. 14 different samples were analyzed. Each experiment with a different sample was replicate two times. We performed a total of 28 experiments and correlated them with 28 experiments of micro-RNA gene expression.

本研究采用的90K康博矩阵（Combimatrix）基因表达微阵列，由三类探针构成：17048条重复探针，以及963条针对Ensembl（版本56）转录本（涵盖蛋白编码基因、假基因及反转录转座子元件）的非重复特异性探针；11363条重复探针，靶向长度介于778 nt与1348 nt之间的UniGene聚类簇（版本38）；剩余的UniGene聚类簇则对应28790条单特异性探针。该微阵列被用于检测14种不同猪组织的基因表达，具体包括左心房、皮肤、肝脏、动脉血白细胞（W_B_A）、静脉血白细胞（W_B_V）、淋巴结、舌、脾脏、骨骼肌、肺、肾脏、胃、脂肪组织及左心室。本研究将上述组织的基因表达与同一组织内的micro-RNA（微小RNA）表达进行关联分析，共分析14份独立样本，每份样本的实验均重复两次，总计完成28次基因表达实验，并将其与28次micro-RNA基因表达实验进行关联比对。