Gene expression profiles of human hematopoietic progenitors [BLUEPRINT hematopoietic progenitors]. Homo sapiens

An optimized whole genome bisulfite sequencing protocol (µWGBS, Farlik et al. 2015 Cell Reports) was used to establish DNA methylation profiles of FACS-purified stem and progenitor cells types of the human blood lineage. Most cell types were sorted from the peripheral blood of three donors each (43 donors total) with eight pools of ten cells, two pools of 50 cells, and one pool of 1000 cells. Additionally, two cell types (HSC, MPP) were sorted from bone marrow, fetal liver, and cord blood; single-cell methylome sequencing (scWGBS, Farlik et al. 2015 Cell Reports) was done for selected cell types (HSC, MPP, CMP, GMP, CLP, MLP0); and gene expression profiling using the Smart-seq2 protocol (Picelli et al. 2013 Nature Methods) was done on all stem/progenitor cell types (HSC, MPP, CMP, MEP, GMP, CLP, MLP0, MLP1, MLP2, MLP3). Overall design: Gene expression was analyzed for stem/progenitor cell types and terminally differentiated cell types of the human blood lineage (HSC, MPP, CMP, GMP, CLP, MLP0, MLP1, MLP2, MLP3). -------------------- The raw sequencing data are available from EBI EGA under a data access agreement. Please contact the authors to access the raw data.优化后的全基因组亚硫酸氢盐测序（whole genome bisulfite sequencing, WGBS）方案（微量全基因组亚硫酸氢盐测序，µWGBS；Farlik等，2015，《Cell Reports》）被用于构建人类血液谱系中经荧光激活细胞分选（fluorescence activated cell sorting, FACS）纯化的干细胞及祖细胞的DNA甲基化图谱。多数细胞类型分别从3名供者的外周血中分选获得（共计43名供者），样本分为8组10细胞混合池、2组50细胞混合池及1组1000细胞混合池。此外，两种细胞类型（造血干细胞（Hematopoietic Stem Cell, HSC）、多能祖细胞（Multipotent Progenitor, MPP））还从骨髓、胎肝及脐带血中分选得到；针对选定的细胞类型（HSC、MPP、共同髓系祖细胞（Common Myeloid Progenitor, CMP）、粒细胞-单核细胞祖细胞（Granulocyte-Monocyte Progenitor, GMP）、共同淋巴系祖细胞（Common Lymphoid Progenitor, CLP）、多淋巴系祖细胞0（MLP0））开展了单细胞甲基化组测序（scWGBS，Farlik等，2015，《Cell Reports》）；同时使用Smart-seq2测序方案（Picelli等，2013，《Nature Methods》）对所有干细胞/祖细胞类型（HSC、MPP、CMP、巨核细胞-红细胞祖细胞（Megakaryocyte-Erythroid Progenitor, MEP）、GMP、CLP、MLP0、MLP1、MLP2、MLP3）进行了基因表达谱分析。 整体实验设计：针对人类血液谱系的干细胞/祖细胞类型及终末分化细胞类型（HSC、MPP、CMP、GMP、CLP、MLP0、MLP1、MLP2、MLP3）开展了基因表达分析。 -------------------- 原始测序数据需遵循数据使用协议，从欧洲生物信息研究所欧洲基因组-表型档案馆（European Bioinformatics Institute European Genome-phenome Archive, EBI EGA）获取，请联系作者以申请原始数据访问权限。