Next Generation Sequencing Quantitative Analysis of altered expression of genes in KRASG12D PDAC tissues

KRAS mutation is the most frequently mutated oncogene, occurring in approximately 90% of patients with PDAC, where KRASG12D is the most common allele in PDAC. Recent studies revealed that KRASG12D mutation caused the aberrant gene expression profile by regulating RNA processing to drive tumor progression (9, 17). However, the role of the KRASG12D mutation in triggering the circRNA process during tumor development remains unknown. Our study aims to explore the genes that regulates circRNA biogenesis in the LN metastasis of PDAC. Overall design: To detect the gees that regulates circRNA biogenesis in LN metastasis of PDAC, the total RNA of KRASG12D PDAC tissues and paired normal adjacent tissues was extracted and the next generation sequencing was conducted to explore the expressing profile difference between PDAC tissues and paired normal adjacent tissues. The result was obtained from three independent experiments.

KRAS突变是最为频发的致癌基因突变，约90%的胰腺导管腺癌（Pancreatic Ductal Adenocarcinoma, PDAC）患者携带该突变，其中KRASG12D是PDAC中最常见的突变等位基因。近期研究显示，KRASG12D突变通过调控RNA加工过程引发异常基因表达谱，进而驱动肿瘤进展（9, 17）。然而，KRASG12D突变在肿瘤发生过程中触发环状RNA（circular RNA, circRNA）生成程序的作用仍未明确。本研究旨在探索胰腺导管腺癌淋巴结转移（Lymph Node Metastasis, LN）中调控环状RNA生物发生的相关基因。总体实验设计：为检测胰腺导管腺癌淋巴结转移中调控环状RNA生物发生的基因，研究人员提取了KRASG12D型PDAC组织及其配对的癌旁正常组织的总RNA，并通过下一代测序（Next Generation Sequencing）开展检测，以分析PDAC组织与配对癌旁正常组织之间的表达谱差异。本研究结果来自3次独立重复实验。