Genome-wide RNA-seq from PARP1 and PRC2 double-depleted MDA-MB-231 cell lines

Purpose: To identify downstream signaling pathways mediated by PARP1 and PRC2 in breast cancer Methods: RNAs isolated from MDA-MB-231 cells depleting PARP1 and EZH2 (PARP1 KO shEZH2) and PARP1 and SUZ12 (PARP1 KO shSUZ12) were analyzed by using an Illumina Nextseq500 Conclusions: Our study represents the first transcriptomic profiles of effects of PARP1 and PRC2 double deficiency in breast cancer mRNAs prepared from wild type, PARP1-EZH2 double-depleted and PARP1-SUZ12 double-depleted MDA-MB-231 cells were analyzed in duplicaters by Illumina Nextseq500.

研究目的：鉴定乳腺癌中由聚腺苷二磷酸核糖聚合酶1（PARP1）与多梳抑制复合体2（PRC2）介导的下游信号通路。
实验方法：提取敲除PARP1并沉默zeste基因增强子同源物2（Enhancer of Zeste Homolog 2, EZH2）（PARP1 KO shEZH2）、以及敲除PARP1并沉默SUZ12多梳复合体亚基（SUZ12 Polycomb Repressive Complex Subunit, SUZ12）（PARP1 KO shSUZ12）的MDA-MB-231细胞的RNA，采用Illumina Nextseq500平台进行测序分析。
研究结论：本研究首次报道了乳腺癌中PARP1与PRC2双缺陷的转录组特征；本研究对野生型、PARP1-EZH2双敲减以及PARP1-SUZ12双敲减的MDA-MB-231细胞制备的mRNA，通过Illumina Nextseq500平台完成了重复实验检测。