Developmental chromatin programs determine oncogenic competence in melanoma [RNA-seq]. Developmental chromatin programs determine oncogenic competence in melanoma [RNA-seq]

Transcriptional profile of human ES-cell (hPSC) derived melanoma cell models Overall design: Human ES cells were differentiated into neural crest (NC), melanoblast (MB) or melanocytes (MC). These were either WT or triple knockout (3xKO) for p53, p16, RB1. A dox-inducible cassette was integrated into the cells to activate the BRAFV600E oncogene upon addition of doxycyline.

人胚胎干细胞（human ES-cell，hPSC）来源的黑色素瘤细胞模型的转录组谱

实验设计概述：将人胚胎干细胞分化为神经嵴（neural crest，NC）、黑素母细胞（melanoblast，MB）或黑素细胞（melanocytes，MC）；上述细胞均分为野生型（WT）与p53、p16、RB1三基因敲除（3xKO）两种基因型。实验中向细胞内整合了多西环素（doxycyline）诱导型基因盒，可在加入多西环素时激活BRAFV600E癌基因。