RNA Sequencing Analysis of Human Ovarian Cancer Cell Line OVCAR5 Treated with Stearic Acid, Oleic Acid, and CAY10566. RNA Sequencing Analysis of Human Ovarian Cancer Cell Line OVCAR5 Treated with Stearic Acid, Oleic Acid, and CAY10566

This study investigates the anti-proliferative effects of long chain fatty acids on cancer cells, focusing on the specific impacts of oleate and stearate. We explored how these fatty acids, prevalent in biological systems, influence cellular processes. Our analysis revealed that the top 10 significantly upregulated Gene Ontology (GO) categories in OVCAR5 cells treated with 50 µM stearate (compared to control cells) are enriched in pathways related to the unfolded protein response (UPR) and endoplasmic reticulum (ER) stress. Overall design: Our experimental design involved the treatment of OVCAR5 cells with various compounds for 24 hours. The treatments included: (i) DMSO (control), (ii) 1 µM CAY10566, (iii) 50 µM stearate with DMSO, (iv) 50 µM stearate with 1 µM CAY10566, (v) 50 µM oleate with DMSO, and (vi) 50 µM oleate with 1 µM CAY10566. After treatment, total RNA was extracted from each sample for RNA sequencing.

本研究旨在探究长链脂肪酸对癌细胞的抗增殖效应，重点聚焦于油酸盐（oleate）与硬脂酸盐（stearate）的具体作用。本研究解析了这类广泛分布于生物系统的脂肪酸对细胞生理过程的调控机制。分析结果显示，经50 μM硬脂酸盐处理的OVCAR5细胞（相较于对照组细胞）中，显著上调的前10个基因本体（Gene Ontology, GO）功能类别，富集于未折叠蛋白反应（unfolded protein response, UPR）与内质网（endoplasmic reticulum, ER）应激相关通路。实验整体设计：本实验将OVCAR5细胞以不同化合物处理24小时，处理组设置如下：(i) 二甲基亚砜（DMSO，对照组）、(ii) 1 μM CAY10566、(iii) 50 μM硬脂酸盐与二甲基亚砜联合处理、(iv) 50 μM硬脂酸盐与1 μM CAY10566联合处理、(v) 50 μM油酸盐与二甲基亚砜联合处理、(vi) 50 μM油酸盐与1 μM CAY10566联合处理。处理结束后，从每份样本中提取总RNA用于RNA测序。