RNA-seq of zinc-replete wild type and zinc-limited wild type, altRP operon deletion mutant and its complement strain in Mycolicibacterium (Mycobacterium) smegmatis

The study was designed to describe the effects of zinc limitation on gene expression in M. smegmatis by comparing the transcriptomes of zinc-replete and zinc-limited wild type. Additionally, the study informs how loss of the altRP operon impacts the transcriptomic changes during zinc limitation by comparing the transcriptomes of zinc-limited ΔaltRP mutant to the zinc-replete and zinc-limited wild type, and to confirm which changes are complemented in the complement strain (ΔaltRP/c). There are 12 samples in total. For each strain (wild type, ΔaltRP and ΔaltRP/c) and condition (ie, zinc-replete and zinc-limited) three biological replicates are analyzed.

本研究旨在通过对比锌充足与锌限制条件下野生型耻垢分枝杆菌（M. smegmatis）的转录组（transcriptome），阐明锌限制对该菌株基因表达的调控效应。此外，本研究通过将锌限制条件下ΔaltRP突变株的转录组与锌充足、锌限制条件下的野生型菌株进行比对，解析altRP操纵子（operon）缺失在锌限制过程中对转录组变化的影响，并验证互补菌株（ΔaltRP/c）中可被恢复的转录组改变。本研究共设置12个样本，针对每株菌株（野生型、ΔaltRP突变株与ΔaltRP/c互补株）及每一种培养条件（锌充足与锌限制），均设置3个生物学重复（biological replicates）进行转录组分析。