Cav3.2+/+ Mouse #405 CA1 EEG recording

A Cav3.2+/+ mouse (internal #405) was implanted with an intrahippocampal electrode for electrohippocampal CA1 EEG recordings. The differential electrode of the TA10ETA-F20 transmitter (Data Science International, DSI, USA, technical specifications: weight 3.9 g, volume 1.9 cc, input voltage range ± 2.5 mV, channel bandwidth (B) 1–200 Hz, nominal sampling rate (f) 1000 Hz (f = 5 B), temperature operating range 34–41 °C) was positioned at the following stereotaxic coordinates: (+)-lead, caudal − 2 mm, lateral of bregma 1.5 mm (right hemisphere), and dorsoventral (depth) 1.5 mm. The epidural reference electrode was positioned on the surface of the cerebellar cortex at the following stereotaxic coordinates: (−)-lead, bregma − 6 mm and lateral of bregma 1 mm (right
hemisphere). The deep tungsten electrodes (FHC, USA) are encapsuled with epoxylite with an
impedance of 50–100 kΩ (measured at 1000 Hz) and a shank diameter of 250 μm. For further details on the Cav3.2 mouse model, animal experimentation approval, anaesthesia, transmitter insertion, stereotaxic electrode implantation, postoperative pain management and recovery, please refer to Arshaad MI et al., 2021. 

EEG data were exported to NeuroScore 3.2.9306-1 (Data Sciences International, DSI, USA). As the NeuroScore file format does not allow free access to the data, we have exported the raw EEG data as asci.-files, so that they can easily be imported in secondary analysis software. Importantly, the exported EEG data represent raw EEG data. No filtering, no artefact detection and/or artefact removal have been carried out. Thus, it is the users responsibility to adequately pretreat the data before further processing and analysis. As described in Arshaad MI et al. (2021), filtering, artefact detection and removal was carried out using NeuroScore before further analysis.

The EEG data are presented here as a zip-folder for size reasons. Four EEG recordings are exported: R1 (a first 24h long-term EEG recording 10 days post transmitter implantation), R2 (a second 24h long-term recording 17 days post transmitter implantation), U1 (a 6h EEG recording following the first urethane injection (800 mg/kg i.p.) at day 18 post transmitter implantation), U2 (a 6h EEG recording  after a second urethane injection (800 mg/kg i.p.) at day 25 post transmitter implantation). In addition, relative activity values are exported for all four recordings. For details on the recording procedure, please refer to Arshaad MI et al. (2021).

本数据集使用内部编号为#405的Cav3.2+/+小鼠，通过海马内电极植入术开展海马CA1区脑电图（Electroencephalogram, EEG）记录。所用TA10ETA-F20型发射机（Data Science International，简称DSI，美国）的差分电极技术参数如下：重量3.9g，体积1.9cc，输入电压范围±2.5mV，通道带宽（B）1~200Hz，标称采样率（f）1000Hz（f=5B），工作温度范围34~41℃。该电极被安置于以下立体定位坐标：正极引线相对于前囟（bregma）尾侧2mm、旁开1.5mm（右侧半球），背腹侧（深度）1.5mm。硬膜外参考电极被安置于小脑皮质表面，坐标为：负极引线相对于前囟尾侧6mm、旁开1mm（右侧半球）。本研究使用的深部钨电极（FHC，美国）采用环氧基树脂（epoxylite）封装，阻抗为50~100kΩ（1000Hz下测得），电极柄直径250μm。关于Cav3.2小鼠模型、动物实验伦理审批、麻醉、发射机植入、立体定位电极植入、术后镇痛与恢复的更多细节，请参阅Arshaad MI等人2021年的研究。

脑电图数据被导入至NeuroScore软件（Data Sciences International，DSI，美国）版本3.2.9306-1中。由于NeuroScore文件格式不支持数据的自由访问，我们将原始脑电图数据导出为ASCII文件，以便后续分析软件可轻松导入。需特别说明的是，本次导出的脑电图数据均为原始数据，未进行任何滤波、伪迹检测或伪迹去除处理。因此，用户需在后续处理与分析前对数据进行恰当的预处理。如Arshaad MI等人（2021）所述，在开展进一步分析前，已通过NeuroScore完成滤波、伪迹检测与去除操作。

考虑到文件大小，本次脑电图数据以ZIP压缩包形式提供。共导出4组脑电图记录：R1为植入发射机后第10天的首次24小时长期脑电图记录；R2为植入后第17天的第二次24小时长期脑电图记录；U1为植入后第18天首次腹腔内注射（intraperitoneal, i.p.）氨基甲酸乙酯（urethane，乌拉坦，800mg/kg）后的6小时脑电图记录；U2为植入后第25天第二次腹腔内注射（800mg/kg）氨基甲酸乙酯后的6小时脑电图记录。此外，还导出了4组记录的相对活动度数值。关于记录流程的详细信息，请参阅Arshaad MI等人2021年的研究。