Nucleotide substitutions within the fold-back arms of the novel pre-miR159 genes and the corresponding pairing in the secondary structure among 42 Phalaenopsis species.

aNucleotide position according to Figure 1. Positions 1–73, 74–139, and 140–214 represent the 5′ fold-back arm, the terminal loop region, and the 3′ fold-back arm, respectively.*Represents increasing stability (new base pairing) of the fold-back structure; ** Represents a minor change in fold-back stability (increase or decrease) without destroying the nucleotide pairing (i.e., A-U→G-U, G-C→G-U, G-U→G-C, or G-U→A-U); *** Represents no change in the fold-back stability or substitutions located in the internal loopsNucleotide substitutions within the fold-back arms of the novel pre-miR159 genes and the corresponding pairing in the secondary structure among 42 Phalaenopsis species.