qPCR results for strains AJ4 and INO2 mutants, targeting the genes EKI1, ERG1, ERG9, ERG20, HNM1, and OLE1

This document presents the qPCR analysis results for strains AJ4, AJ4 Ino2pI263V, AJ4 Ino2pR86H, and AJ4 Ino2pI263V, R86H), each analyzed in triplicate (A, B, C), for the genes EKI1, ERG1, ERG9, ERG20, HNM1, and OLE1, using ACT1 and RDN18-1 (18S) as reference genes. Calculations normalized to t0 are included, along with statistical analyses and the oligonucleotide sequences used. This analysis was performed to verify the role of the two amino acid substitutions in INO2 of AJ4 in ethanol tolerance, as identified in the study Identification of a crucial INO2 allele for enhancing ethanol resistance in an industrial fermentation strain of Saccharomyces cerevisiae.本数据集收录了菌株AJ4、AJ4 Ino2pI263V、AJ4 Ino2pR86H及AJ4 Ino2pI263V/R86H的实时定量聚合酶链反应（qPCR）分析结果。上述菌株针对基因EKI1、ERG1、ERG9、ERG20、HNM1与OLE1开展检测，每个菌株均设置3次平行重复实验（标记为A、B、C），并以ACT1和RDN18-1（18S rRNA）作为内参基因。本数据包含以t0为基准的归一化计算结果、统计分析数据以及所用寡核苷酸引物序列。 本分析旨在验证AJ4菌株INO2基因中两处氨基酸替换在乙醇耐受性中的调控作用，该发现源自研究《酿酒酵母工业发酵菌株中增强乙醇抗性的关键INO2等位基因的鉴定》。