MIGen_ExS: PROCARDIS

The Precocious Coronary Artery Disease (PROCARDIS) study is an international, multicenter case-control study aimed at discovering the genetic contributors to premature coronary artery disease. All exome sequencing was performed at the Broad Institute of Harvard and MIT; samples sequence capture was performed using Agilent SureSelect Human All Exon Kit v2 and sequencing was performed on an Illumina HiSeq 2000 or 2500.]]>
Cases and controls were recruited from the United Kingdom. Cases were considered eligible for the study if premature coronary artery disease was present. This was defined as myocardial infarction (MI), unstable or stable angina, or coronary revascularization that occurred before age 66. 80% of cases had a sibling with a history of MI or acute coronary syndrome before age 66. Controls were matched by age and gender and recruited from the same centers simultaneously. Controls were eligible for the study if they had no personal or sibling history of MI, unstable or stable angina, or coronary revascularization before the age of 66.]]>

早发性冠心病（Precocious Coronary Artery Disease，缩写PROCARDIS）研究是一项国际性多中心病例对照研究，旨在探究早发性冠心病的遗传致病因素。所有外显子组测序（exome sequencing）均在哈佛-麻省理工布洛德研究所（Broad Institute of Harvard and MIT）完成；样本序列捕获采用安捷伦SureSelect人类全外显子组捕获试剂盒v2（Agilent SureSelect Human All Exon Kit v2），测序工作在Illumina HiSeq 2000或2500测序平台上开展。
病例与对照均招募自英国。符合本研究入组标准的病例需确诊为早发性冠心病，其定义为66岁前发生心肌梗死（myocardial infarction，简称MI）、不稳定型或稳定型心绞痛，或接受过冠状动脉血运重建术。本研究中80%的病例拥有一名66岁前存在心肌梗死或急性冠脉综合征病史的同胞。
对照人群按照年龄与性别进行匹配，且同步在相同研究中心招募。符合本研究入组标准的对照需满足：66岁前无个人或同胞的心肌梗死、不稳定型或稳定型心绞痛，或冠状动脉血运重建病史。