Discovery of extracellular vesicles derived miR-185-5p in patient's plasma as an indicator for advanced adenoma and malignant tumor of colon

We attempted to screen and identify new plasma EV delivered microRNA can simultaneously diagnose AA, early CRC and advanced CRC in healthy people. We employed microRNA deep sequencing assay for the primary screen and performed TaqMan microRNA assay for further test and validation. Through stepwise screen and validation in two independent cohorts (cohort I: n=75, cohort II: n=104), we demonstrated that plasma EV-delivered miR-185-5p is a potential indicator of AA and CRC in Chinese patients. In the supplementary phase, we also preliminarily proved that miR-185-5p can promote the development of CRC in vivo and in vitro. Overall design: Comparative miRNA expression profiling analysis of miRNA-seq data for plasma extracellular vesicles in healthy donor, advanced adenoma, and colorectal cancer patients.

本研究旨在筛选并鉴定可在健康人群队列中同时区分腺瘤性息肉（Adenomatous Polyp, AA）、早期结直肠癌（Colorectal Cancer, CRC）与进展期结直肠癌患者的新型血浆细胞外囊泡（Extracellular Vesicles, EV）携带的微小RNA。本研究采用微小RNA深度测序技术开展初步筛选，并通过TaqMan微小RNA检测试剂盒完成后续验证与确证实验。通过对两个独立队列（队列I：n=75；队列II：n=104）开展分步筛选与验证，本研究证实血浆EV携带的miR-185-5p可作为中国人群中AA与CRC的潜在检测标志物。在补充实验阶段，本研究还初步验证了miR-185-5p可在体内与体外环境中促进CRC的发生发展。整体实验设计：针对健康供体、进展性腺瘤患者及结直肠癌患者的血浆细胞外囊泡，开展基于miRNA-seq数据的比较性miRNA表达谱分析。