ExtFig9A_RACE_PR8-NS_R1-R2-R3_LG290-292-293.sgd
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Xrn1 ko A549 cells were infected with WT or PR8-PA(∆X), or mock infected. RNA was extracted to run 5’ RACE using primers ~150-200 nt downstream of GCUG sites that are predicted to be inside a hairpin loop within viral mRNAs (in segment 4 or 8 as indicated)
将Xrn1基因敲除的A549细胞分别以野生型(WT)、PR8-PA(∆X)毒株感染,同时设置空白感染对照。提取细胞总RNA后,设计针对被预测存在于病毒mRNA发夹环结构内的GCUG位点下游约150~200 nt区域的引物,开展5' 快速扩增cDNA末端(5’ RACE)实验,上述GCUG位点对应第4或第8基因组节段(如标注所示)
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Gaglia, Marta创建时间:
2023-01-19



