table 9_Cerebrospinal Fluid Inflammatory Cytokine Levels in Patients With Major Psychiatric Disorders: A Multiplex Immunoassay Study.xlsx

Aim: Accumulating evidence suggests that neural inflammation plays an important role in psychiatric disorders. We aimed to identify inflammatory cytokines involved in the pathophysiology of such disorders by quantifying them in cerebrospinal fluid (CSF) samples from a large sample of patients with major psychiatric disorders and healthy controls.
Methods: The subjects included 94 patients with schizophrenia, 68 with bipolar disorder, 104 with major depressive disorder, and 118 healthy controls, matched for age, sex, and ethnicity (Japanese). Lumbar puncture was performed to collect these CSF samples. A multiplex immunoassay was then performed to measure CSF cytokine levels using magnetic on-bead antibody conjugation for 19 inflammatory cytokines.
Results: CSF interferon-β level was significantly higher in total psychiatric patients than in healthy controls (corrected p = 0.000029). In diagnostic group comparisons, CSF interferon-β level was significantly higher in patients with schizophrenia, or bipolar disorder (corrected p = 0.000047 or 0.0034) than in healthy controls.
Conclusion: We present novel evidence that CSF IFN-β level showed prominent statistical differences between psychiatric groups and healthy controls. This suggests IFN-β as the most important player among the 19 cytokines tested here in the inflammation-related pathophysiology of major psychiatric disorders.

研究目的：日益增多的研究证据表明，神经炎症在精神疾病中发挥着关键作用。本研究旨在通过对大样本重型精神疾病患者与健康对照者的脑脊液（cerebrospinal fluid, CSF）样本进行炎性细胞因子定量检测，筛选参与此类疾病病理生理过程的关键炎性细胞因子。
研究方法：研究对象包括94例精神分裂症患者、68例双相情感障碍患者、104例重度抑郁症患者以及118名健康对照者，所有受试者在年龄、性别及种族（日本人群）维度上均匹配。通过腰椎穿刺术采集脑脊液样本，随后采用磁珠抗体偶联的多重免疫分析法，对19种炎性细胞因子的脑脊液水平进行定量检测。
研究结果：与健康对照者相比，所有精神疾病患者的脑脊液干扰素-β（interferon-β, IFN-β）水平均显著升高（校正后P=0.000029）。在诊断亚组间比较中，精神分裂症患者或双相情感障碍患者的脑脊液干扰素-β水平同样显著高于健康对照者（校正后P分别为0.000047和0.0034）。
结论：本研究提供了新的证据，表明精神疾病患者各组与健康对照者的脑脊液IFN-β水平存在显著统计学差异。这提示在本次检测的19种细胞因子中，IFN-β是与重型精神疾病炎症相关病理生理过程关联最为密切的核心因子。