MOESM5 of Global discovery of small RNAs in the fish pathogen Edwardsiella piscicida: key regulator of adversity and pathogenicity

Additional file 5. The expression of Hfq-dependent sRNAs were identified by quantitative real time PCR. Five upregulated sRNAs (left) and 14 downregulated sRNAs (right) identified by RNA-sequencing were checked by qRT-PCR. The experiment was performed three times, and values are shown as mean ± SEM. *P &lt; 0.05, **P &lt; 0.01.附加文件5。本文件展示了通过实时定量聚合酶链反应（quantitative real-time PCR，qRT-PCR）鉴定Hfq依赖型小RNA（small RNA，sRNA）表达水平的实验结果。经RNA测序（RNA-sequencing，RNA-seq）筛选得到的5个上调表达sRNA（左侧）与14个下调表达sRNA（右侧），均通过qRT-PCR完成验证。本实验重复三次，数据以平均值±标准误（standard error of the mean，SEM）表示。*P < 0.05，**P < 0.01。