Halobacterium NRC-1, EMS Stress Response

100mM EMS was added to mid-log phase Halobacterium NRC-1 cultures. After constant stress of EMS for 30 minutes, cultures were spun down and pellets were re-suspended in same volume of GN101 media. Cultures were then harvested by centrifugation and pellets were snap frozen on a dry-ice ethanol bath. Samples for RNA preparation were collected during recovery time points at 0, 10, 20, 30, 40, 60 and 120 minutes. Keywords: stress response, dose response 16 samples (8 samples from EMS perturbed and 8 non-perturbed controls) were analyzed on replicate arrays (dye-flips) all against the same standard reference sample.

将100mM浓度的乙基甲磺酸（Ethyl Methanesulfonate，EMS）添加至处于对数生长期中期的盐杆菌NRC-1（Halobacterium NRC-1）培养液中。经持续EMS胁迫处理30分钟后，将培养液离心以收集菌体沉淀，随后将沉淀重悬于等体积的GN101培养基中。再次通过离心收获菌体，并将菌体沉淀置于干冰乙醇浴中快速速冻。用于RNA制备的样本于恢复阶段的0、10、20、30、40、60及120分钟时间点采集。关键词：胁迫响应、剂量响应。共计16个样本（其中8个为EMS胁迫处理样本，8个为未受扰动的对照样本），所有样本均采用染料翻转重复芯片进行分析，并均与同一标准参考样本进行比对。