Effect of high temperature on Arabidopsis thaliana root tips. Effect of high temperature on Arabidopsis thaliana root tips

Plant roots located in the upper soil layers are prone to experience high temperatures. To gain insight into the effect of high temperature on root development and functioning, we exposed five-day-old Arabidopsis thaliana seedlings grown on agar plates to 30 °C for 48 hours, and compared the gene expression profile in the root tip with that from seedlings that remained at 22 °C. Overall design: Seedlings were grown on agar plates for 5 days at 22 °C before start of the treatment. Control treatment was 48 hours at 22 °C, high temperature treatment was 48 hours at 30 °C. At the end of the treatment, 1-mm long tips of the roots were collected. 4 biological replicates were used from each treatment. A replicate consisted of pooled tissue from 50 seedlings (grown on 5 plates, 10 seedlings/plate).

生长于上层土壤中的植物根系易遭遇高温环境。为深入探究高温对根系发育与生理功能的影响，本研究将在琼脂平板上培养5天的拟南芥（Arabidopsis thaliana）幼苗置于30℃条件下处理48小时，并与始终维持22℃生长的对照组幼苗的根尖基因表达谱进行对比分析。实验总体设计：处理开始前，所有幼苗均在22℃环境下于琼脂平板上培养5天；对照组为22℃下持续培养48小时，高温处理组则在30℃条件下培养48小时。处理结束后，采集长度为1mm的根尖组织。每组处理设置4次生物学重复，单次生物学重复包含50株幼苗的混合组织（分置于5个平板培养，每个平板接种10株幼苗）。