Transciptome profiling of RNaseIII null cells reconstituted with Drosha. Homo sapiens

To assess the impact of Drosha reconstitution on the overall transcriptome of RNaseIII null cells. Overall design: We utilized CRISPR techology to engineer an RNaseIII null cell line using the NoDice 293T (PMID: 24757167) as the parental cell line. mRNA profiles of RNaseIII null cells were transfected with GFP plus empty plasmid or GFP-Drosha for 3 days.

为评估Drosha重构对核糖核酸酶III（RNaseIII）缺陷型细胞的整体转录组的影响。实验设计：本研究以NoDice 293T（PMID: 24757167）作为亲本细胞系，利用CRISPR技术构建核糖核酸酶III缺陷型细胞系；将该细胞系分为两组，分别转染GFP空质粒与GFP-Drosha融合质粒，转染后培养3天，随后采集两组细胞的mRNA表达谱。