Table 2.2 Guide 3 off-target locations

Guide RNA sequence and in silico predicted off-target locations as determined using an online tool (http://crispr.mit.edu). Pairs of bidirectional sgRNAs were used in conjunction with a mutant Cas9 D10A nickase to further reduce the chance of off-target events.本数据集包含向导RNA（Guide RNA）序列，以及通过在线工具（http://crispr.mit.edu）计算机模拟预测得到的脱靶位点。本研究采用双向单引导RNA（single guide RNA, sgRNA）对，与突变型Cas9 D10A切口酶联用，以进一步降低脱靶事件的发生概率。