RNA-sequencing analysis for gene expression profiles affected by CASC9 knockdown. RNA-sequencing analysis for gene expression profiles affected by CASC9 knockdown

Genome-wide association studies of tumor samples have identified a large number of lncRNAs associated with various types of cancer including colorectal cancer. To elucidate the mechanism by which CASC9 regulates colorectal cancer cell growth, RNA-sequencing was performed to analyze the gene expression profile affected by CASC9 knockdown. A total of 249 significantly upregulated genes and 491 significantly downregulated genes (absolute fold change ≥ 2, P < 0.05) were found in CASC9 knockdown HCT-116 cells. Overall design: Comparing gene expression profiles of HCT-116 cells transfected with CASC9 GapmeR (GapC9) and the control GapmeR (GapNC)

针对肿瘤样本开展的全基因组关联研究（Genome-wide association studies）已鉴定出大量与结直肠癌等多种癌症相关的长链非编码RNA（long non-coding RNAs, lncRNAs）。为阐明CASC9调控结直肠癌细胞生长的分子机制，本研究通过RNA测序（RNA-sequencing）分析了CASC9敲低所影响的基因表达谱。在CASC9敲低的HCT-116细胞中，共筛选得到249个显著上调基因与491个显著下调基因（绝对折叠变化≥2，P<0.05）。实验整体设计：对比转染CASC9 GapmeR（GapC9）与阴性对照GapmeR（GapNC）的HCT-116细胞的基因表达谱。