Effects of SPOP on gene expression in prostate cancer

SPOP is a ubiquitin ligase adaptor frequently mutated in prostate cancer. It is involved in ubiquitination and degradation of substrate proteins. We examined the impact of wild-type and mutant SPOP on the transcriptional profile of prostate cancer cells. We cloned several naturally occurring (in human prostate cancer) SPOP mutants and expressed the corresponding constructs in prostate cancer cells. Our experimental conditions were: Human prostate cancer cells (LNCaP-Abl), transfected with control vector, SPOP-wt, and any of the following mutants: SPOP-F102C, SPOP-F133V, SPOP-F133L (2-4 biological replicates each). We analyzed their gene expression profiles for differences induced by SPOP-wt vs SPOP-mutant.

SPOP是一种泛素连接酶衔接蛋白（ubiquitin ligase adaptor），在前列腺癌中频发突变，参与底物蛋白的泛素化与降解过程。本研究旨在探究野生型与突变型SPOP对前列腺癌细胞转录组谱的影响。我们克隆了数株在人类前列腺癌中自然发生的SPOP突变体，并将对应的表达载体转染至前列腺癌细胞内。实验模型与分组如下：以人类前列腺癌细胞LNCaP-Abl为研究对象，分别转染空载体、SPOP野生型（SPOP-wt）以及下述任一突变体：SPOP-F102C、SPOP-F133V、SPOP-F133L，每组设置2-4次生物学重复。后续我们对各组的基因表达谱进行分析，以比较SPOP野生型与突变型所诱导的基因表达差异。