Targeted delivery of the PKMYT1 inhibitor RP-6306 mediates PANoptosis in pancreatic cancer via mitotic catastrophe

MiaPaCa-2 cells were treated with RP-6306 or RP-6306+Gemcitabine or DMSO for 48 h. Then, total RNA was extracted from cells using TRIzol (Invitrogen). Library construction and sequencing were performed by LC-Bio Technology (Hangzhou, China). The average insert size for the final cDNA library was 300±50 bp. At last, we performed the 2×150bp paired-end sequencing (PE150) on an illumina Novaseq™ 6000 (LC-Bio Technology CO., Ltd., Hangzhou, China) following the vendor's recommended protocol.

将MiaPaCa-2细胞分别采用RP-6306、RP-6306联合吉西他滨（Gemcitabine）或二甲基亚砜（DMSO）处理48小时。随后使用TRIzol（Invitrogen）从细胞中提取总RNA。文库构建与测序由杭州LC-Bio生物科技有限公司（LC-Bio Technology, Hangzhou, China）完成。最终cDNA文库的平均插入片段长度为300±50 bp。最后，我们依照厂商推荐的实验流程，在Illumina NovaSeq™ 6000测序平台（杭州LC-Bio科技有限公司，LC-Bio Technology CO., Ltd., Hangzhou, China）上开展了2×150 bp双末端测序（PE150）。