Summary and assessment of the data set

Juniperus squamata, a tetraploid species which is widely distributed in Asian mountains, represents the largest genus, Juniperus, in Cupressaceae. Single-molecule real-time sequencing was used to obtain full-length transcriptome of Juniperus squamata. The full-length transcriptome was corrected with Illumina RNA-seq data from the same individual. A total of 47, 860 non-redundant full-length transcripts, N50 of which was 2, 839, were obtained. Simple sequence repeats for Juniperus squamata were also identified. This data presents the first comprehensive transcriptome characterization of Cupressaceae species, and provides an important reference for researches on the genomic evolutionary history of Cupressaceae plants and even conifers in the future.方枝柏（Juniperus squamata）是一种广泛分布于亚洲山地的四倍体物种，其所属的刺柏属（Juniperus）为柏科（Cupressaceae）中最大的属。本研究采用单分子实时测序（Single-Molecule Real-Time Sequencing）技术获取了方枝柏的全长转录组，并利用同一单株的Illumina RNA测序（Illumina RNA-seq）数据对所得全长转录组进行校正。最终共获得47860条非冗余全长转录本，其N50值为2839。本研究还鉴定得到了方枝柏的简单序列重复（Simple Sequence Repeats）位点。本数据集首次完成了柏科物种的全面转录组特征解析，可为未来柏科乃至针叶树（Conifers）的基因组演化历史相关研究提供重要参考依据。