EBNA-2 target genes

Two cell lines, BL41K3 and BJABK3, were chosen to perform a comprehensive screen for EBNA-2 target genes. Both cell lines are EBV negative B-cell lines and express a chimeric EBNA-2 protein fused to the hormone binding domain of the estrogen receptor (ER/EBNA-2). The cells are propagated in the absence of hormone 31,36. Estrogen stimulation activates ER/EBNA-2, which in turn activates well known EBNA-2 target genes like CD21. BL41K3 and BJABK3 were cultured in estrogen supplemented medium for 24h and gene expression profiles were established for unstimulated and stimulated cells. Keywords: Transcription factor target identification BJABK3: 3 biological replicates, for unstimulated and EBNA-2 induced cells, resp. BL41K3: 2 biological replicates, for unstimulated and EBNA-2 induced cell, resp.

本研究选取BL41K3与BJABK3两种细胞系，针对EBNA-2靶基因开展全面筛选。两种细胞系均为EB病毒（Epstein-Barr virus, EBV）阴性的B细胞系，且均表达融合了雌激素受体（estrogen receptor, ER）激素结合结构域的嵌合EBNA-2蛋白（ER/EBNA-2）。细胞在无激素条件下进行传代培养[31,36]。雌激素刺激可激活ER/EBNA-2，进而激活CD21等经典EBNA-2靶基因。将BL41K3与BJABK3置于添加雌激素的培养基中培养24小时，分别构建未刺激与雌激素刺激状态下细胞的基因表达谱。
关键词：转录因子靶标鉴定
BJABK3：未刺激组与EBNA-2诱导组分别设置3次生物学重复；
BL41K3：未刺激组与EBNA-2诱导组分别设置2次生物学重复。