Haemophilus influenzae 86-028NP

Transcriptome analysis of NTHi 86-028NPrpsL, NTHi 86-028NPrpsL∆fur, and NTHi 86-028NPrpsL∆fur(pT-fur) strains Nontypeable Haemophilus influenzae (NTHi) is a commensal microorganism of the normal human nasopharyngeal flora, yet also an opportunistic pathogen of the upper and lower respiratory tracts. Changes in gene expression patterns in response to host microenvironments are likely critical for survival. One such system of gene regulation is the ability to carefully regulate iron uptake. A central regulatory system that controls iron uptake, mediated by the ferric uptake regulator Fur, is present in multiple bacteria, including NTHi. To understand the regulation of iron homeostasis in NTHi, fur was deleted in the NTHi strain 86-028NPrpsL. Using RNA-Seq, we identified both protein-encoding and small RNA genes whose expression was repressed or activated by Fur. Overall design: These data comprise transcriptional anaylses of an rpsL mutant of 86-028NP, an isogenic fur mutant of 86-028NPrpsL and a complemented fur mutant strain. All strains were grown in defined medium containing 10 µg/ml human hemoglobin to mid-log phase. Cells were then harvested and RNA extracted. A total of three biological replicates were generated for these analyses. Analysis of transcriptomes using the Illumina HiSeq 2000 of three strains of nontypeable Haemophilus influenzae which include NTHi 86-028NPrpsL, NTHi 86-028NPrpsL∆fur, and NTHi 86-028NPrpsL∆fur(pT-fur) strains. For each strain three biological replicates were analyzed

NTHi 86-028NPrpsL、NTHi 86-028NPrpsL∆fur及NTHi 86-028NPrpsL∆fur(pT-fur)菌株的转录组分析

非分型流感嗜血杆菌（Nontypeable Haemophilus influenzae, NTHi）是人类正常鼻咽菌群的共生微生物，同时也是上下呼吸道的条件致病菌。宿主微环境诱导的基因表达模式改变，对其生存至关重要。其中一类关键基因调控系统，是精准调控铁摄取的能力。包括NTHi在内的多种细菌中，均存在由铁摄取调节因子（ferric uptake regulator, Fur）介导的核心铁摄取调控系统。为解析NTHi中铁稳态的调控机制，研究人员在NTHi菌株86-028NPrpsL中敲除了fur基因。通过RNA测序（RNA-Seq），我们鉴定出了受Fur激活或抑制的蛋白编码基因与小RNA（small RNA）基因。

总体实验设计：本数据集包含三类菌株的转录组分析：86-028NP的rpsL突变株、86-028NPrpsL的同基因fur突变株，以及互补型fur突变株。所有菌株均在添加10 μg/ml人血红蛋白的限定培养基中培养至对数中期，随后收集细胞并提取总RNA。本分析共设置3个生物学重复。

本研究利用Illumina HiSeq 2000平台，对3株非分型流感嗜血杆菌进行转录组分析，所涉菌株分别为NTHi 86-028NPrpsL、NTHi 86-028NPrpsL∆fur及NTHi 86-028NPrpsL∆fur(pT-fur)。每株菌株均设置3个生物学重复进行分析。