Optimization of Hydrolase Production (Dataset).pdf

The saccharification of biomass with hydrolases from fungi is considered as an efficient and sustainable approach to obtain fermentable sugars. The expression of these hydrolytic enzymes by fungi is mainly by a substrate-induced mechanism, which depends on prevailing environmental conditions. <i>Trametes polyzona, </i>a white rot fungus, is regarded as one of the most efficient producers of hydrolases for the hydrolysis of biomass. This study sought to examine and optimize cultivation conditions for the efficient production of hydrolases by <i>Trametes polyzona </i>(<i>T. polyzona</i>)<i>. </i>Hydrolase production by <i>T. polyzona</i> was examined using parametric optimization. Crude enzyme extracts obtained under varying cultivation conditions were profiled for endoglucanase, betaglucosidase, exoglucanase, xylanase and amylase activities to determine the optimal conditions for hydrolase production. Maximum hydrolase production was achieved by solid-state cultivation of <i>T. polyzona</i> on cassava peels at a moisture content of 60%, pH 6, supplemented with 0.3% urea and 0.03% Mg²⁺, and incubated at 30 ^oC for 14 days. A solid to liquid ratio of 1:2 was found to be ideal for the solid-liquid extraction of enzymes. This study therefore proves that optimization of cultivation conditions is an efficient approach to enhance the production of hydrolases required to breakdown lignocellulosic biomass by <i>T. polyzona.</i>

利用真菌源水解酶进行生物质糖化，是获取可发酵糖的高效且可持续的途径。真菌合成此类水解酶的表达机制主要为底物诱导型，且该机制受所处环境条件的影响。多带栓菌（Trametes polyzona）是一种白腐真菌，被认为是用于生物质水解的高效水解酶生产菌种之一。本研究旨在优化多带栓菌高效合成水解酶的培养条件，并通过参数优化手段探究该菌的水解酶合成情况。针对不同培养条件下获得的粗酶提取物，本研究检测其内切葡聚糖酶、β-葡萄糖苷酶、外切葡聚糖酶、木聚糖酶与淀粉酶活性，以确定水解酶合成的最优培养条件。多带栓菌以木薯皮为底物进行固态发酵时，可获得最高的水解酶产量：培养条件为含水量60%、pH值6，添加0.3%尿素与0.03%镁离子（Mg²+），于30℃下孵育14天。固液比1:2被证实为酶的固液提取的最优比例。综上，本研究证实，优化培养条件可有效提升多带栓菌合成降解木质纤维素生物质所需水解酶的产量。