Analysis of Expressed sequence tag in maritime pine (Pinus pinaster Ait.): drought stress. Pinus pinaster

cDNA libraries were generated using total RNA extracted from quiescent buds harvested on 2 years old maritime pine (fast or low growing family) in well watered or drought stress conditions. The libraries were subjected to high throughput sequencing on a 454 Titanium platform.

本研究从分别在正常浇水或干旱胁迫条件下采集的、两年生滨海松（速生或慢生家系）的休眠芽中提取总RNA，以此构建互补DNA（cDNA）文库；随后采用454 Titanium测序平台对该文库开展高通量测序。