PMACS-76

<b>Project title</b>: PMACS-76<b>Grant Information:</b> AHA Grant 9TPA34910007, Regulatory role of pericardial macrophages in myocardial infarction<b>Project Description:</b>· Animal Model: Myocardial Infarction· Animal Age: 8-12 Weeks· Animal Sex: Male· Animal Strain: GATA6^Venus Reporter or WT· Number: 7 mice· Groups: 4 Venus infarction, 3 Venus sham surgery, WT received sham surgery· Experimental Conditions: Mice were given MI via LAD ligation. Animals were sacrificed at day 3 post-infarction or sham surgery. Heats were collected. Results were analyzed via flow cytometry examining for Venus reporter expression<b>Code/Scripts: </b>N/A<b>Licenses: </b>N/A<b>项目标题</b>: PMACS-76 <b>资助信息</b>: 美国心脏协会（American Heart Association, AHA）资助项目编号9TPA34910007，主题为“心包巨噬细胞在心肌梗死中的调控作用” <b>项目描述</b>: · 动物模型：心肌梗死（Myocardial Infarction, MI）模型 · 动物周龄：8~12周 · 动物性别：雄性 · 动物品系：GATA6^Venus报告基因小鼠或野生型（Wild Type, WT）小鼠 · 实验动物数量：7只小鼠 · 实验分组：4只GATA6^Venus报告基因小鼠接受心肌梗死造模，3只该品系小鼠接受假手术，野生型小鼠仅接受假手术 · 实验流程：通过左前降支（Left Anterior Descending artery, LAD）结扎构建小鼠心肌梗死模型；于造模或假手术后第3天处死动物，采集心脏组织；采用流式细胞术检测Venus报告基因的表达以分析实验结果 <b>代码/脚本</b>: 无 <b>许可协议</b>: 无