miR-21-5p-driven gene expression in human colorectal DLD-1 cells.

Gene expression analysis of stable miR-21-5p-overexpressing DLD-1 cells (DLD-1-miR-21) and its control cells (DLD-1-miR-Vec). To establish stable miR-21-overexpressing DLD-1 (DLD-1-miR-21) and corresponding vector-overexpressing (DLD-1-miR-Vec) cell lines, parental DLD-1 cells were transfected with a plasmid encoding human miR-21 or its control vector (pCMV-MIR) using the PolyJet transfection reagent. Stable clones were selected with 1 mg/mL G418 for 2 months. Single clones were selected based on expression of the green fluorescent protein (GFP) reporter and then pooled together for subsequent experiments.

本数据集针对稳定过表达miR-21-5p的DLD-1细胞（DLD-1-miR-21）及其对照细胞（DLD-1-miR-Vec）开展基因表达分析。为构建稳定过表达miR-21的DLD-1细胞系（DLD-1-miR-21）与对应的空载体过表达细胞系（DLD-1-miR-Vec），研究人员采用PolyJet转染试剂，将编码人源miR-21的质粒或其对照载体（pCMV-MIR）转染至亲本DLD-1细胞中。随后以1 mg/mL的G418筛选稳定克隆，筛选周期为2个月。基于绿色荧光蛋白（green fluorescent protein，GFP）报告基因的表达水平筛选单克隆细胞株，将筛选得到的单克隆混合后用于后续实验。