Gene expression in 'Newton' wheat in response to greenbug infestation and Cereal Yellow Dwarf Virus infection

This factorial RNAseq study of gene expression in wheat leaves was performed to document responses to infestation with two virulent biotypes (B and H) of greenbug (Schizaphis graminum (Rondani)) with and without carried Cereal Yellow Dwarf Virus (CYDV-RPV) over a 20-day time course. It characterized changes in gene expression related to greenbug feeding, CYDV disease, and their interaction. The wheat (cv. 'Newton') had not been vernalized and therefore did not flower during the experiment, but there was no control for aging in the absence of greenbugs or CYDV over the time course. These plants were the substrate of the greenbugs sampled in SRA bioproject PRJNA981508.本析因转录组测序（RNAseq）研究以小麦叶片为对象，旨在阐明其在20天时间序列中，受两种分别携带/不携带谷类黄矮病毒（Cereal Yellow Dwarf Virus, CYDV-RPV）的强毒麦二叉蚜（Schizaphis graminum (Rondani)）生物型B与H侵染后的基因表达响应。该研究表征了与麦二叉蚜取食、CYDV病害及其互作相关的基因表达变化。本试验所用小麦品种为cv. 'Newton'，未经过春化处理，因此试验全程未抽穗，但未设置无麦二叉蚜侵染与CYDV感染情况下的植株衰老对照。这些植株即为SRA生物项目PRJNA981508中所采样麦二叉蚜的寄主材料。