Analysis of the collagen fibers on autopsied patients’ uterus with the Acquired Immunodeficiency Syndrome

Abstract Objectives: to compare the percentage of collagen fibers in the autopsied women’s uterine body and cervix with and without the Acquired Immunodeficiency Syndrome (Aids). Methods: 30 autopsied women’s medical files were selected from 1988 to 2013. 30 fragments of the uterine body and 30 cervix were collected and then divided into two groups, 15 with Aids and 15 without, The quantification of the collagen fibers of the uterine body and cervix was performed on slides stained with picrosirius, using the KS-300® system. Results: the percentage of collagen fibers was lower for cervix (U=336544; p=0.001) and higher for the uterine body (U=308726,5; p=0.004) in the retroviral group when compared to the group without the disease. The percentage was higher for cervix than the uterine body in the group with Aids (t=0,4793; p=0.0031). the same result was found in the group without Aids (t=2,397; p=0.0637). Conclusions: the increase in the percentage of collagen fibers in the uterine body of women with Aids’ indicates an immune response for viral infection and reveals a failure in keeping the infection restricted to the cervix. The interpretation of the histochemical and morphometric parameters can be useful in the diagnosis associated to HIV infection, contributing for clinical improvement and life expectancy.

摘要 研究目的：比较伴与不伴获得性免疫缺陷综合征（Acquired Immunodeficiency Syndrome, AIDS）的尸检女性子宫体与宫颈的胶原纤维占比。方法：选取1988年至2013年间的30份尸检女性病历资料，采集子宫体与宫颈组织标本各30份，分为两组：伴AIDS组与非AIDS组，每组各15份。采用天狼星红染色法对标本切片进行染色，并借助KS-300®系统对子宫体与宫颈的胶原纤维进行定量分析。结果：相较于非AIDS组，AIDS组患者宫颈的胶原纤维占比更低（U=336544；p=0.001），而子宫体的胶原纤维占比更高（U=308726.5；p=0.004）。在AIDS组中，宫颈的胶原纤维占比高于子宫体（t=0.4793；p=0.0031）；非AIDS组亦得到相同结果（t=2.397；p=0.0637）。结论：AIDS患者子宫体胶原纤维占比升高，提示机体针对病毒感染产生免疫应答，同时表明感染未能被局限于宫颈部位。对组织化学与形态计量学参数的解读，可辅助HIV感染相关疾病的诊断，有助于改善临床预后与延长患者预期寿命。