Effect of flg22 peptide on Arabidopsis seedling roots with endodermis-expressed FLS2 in fls2 mutant background

To compare transcriptional outputs of FLS2 and SGN3 pathways solely within the endodermis, we conducted comparative RNAseq analysis of pCASP1::FLS2 fls2 and wild-type seedling roots treated by flg22 and CIF2 respectively, at three timepoints (30, 120, 480 min) (CIF2 responses data previously published by Fujita et al 2020). The receptor mutant controls sgn3 and fls2, displayed minimal gene responses to respective peptides. Comparing flg22 treatment to H2O treatment at 3 time points (30, 120, 480 min) on pCASP1::FLS2-GFP fls2 (endodermis-expressed FLS2) to assess endodermis-specific FLS2 immune responses in 5-day-old seedling roots. The fls2 receptor mutant is used as a control.

为仅在内皮层中比较FLS2与SGN3通路的转录输出，我们分别对经flg22与CIF2处理的pCASP1::FLS2 fls2及野生型幼苗根系开展了比较RNA测序（RNAseq）分析，实验设置3个时间节点（30、120、480分钟），其中CIF2应答相关数据此前已由Fujita等人于2020年发表。受体突变体对照sgn3与fls2仅对各自对应的肽类产生极微弱的基因表达应答。
为评估5日龄幼苗根系内皮层特异性FLS2免疫应答，我们还在3个时间节点（30、120、480分钟）下，对比了pCASP1::FLS2-GFP fls2（内皮层表达的FLS2，融合绿色荧光蛋白(GFP)）经flg22处理与H2O处理后的转录组差异，其中fls2受体突变体作为实验对照。