GNPS - Arterio-venous metabolomics for postprandial inter-organ flux analysis

Yucatan minipigs, fasted overnight, were previously fed either a control or a Western diet (high-fat, high-sucrose). They received 23.7ml/kg of their body weight in liquid diet via intra-gastric tubing, with blood samples collected 120 minutes post-administration. For sampling, catheters were inserted into the femoral artery and vein. Blood was drawn from the hepatic, portal, renal, splenic, and inferior mesenteric veins, the lateral auricular vein, the aorta, and the coronary sinus using a 23G butterfly needle attached to a 1 mL syringe. The external jugular vein and the left and right ventricles were sampled using a 27G needle connected to a 1 mL syringe. All organ blood samples were taken within 5-10 minutes of the initial sampling. Tissue samples were collected post-terminal surgery.

尤卡坦小型猪（Yucatan minipigs）经一夜空腹处理，此前分别饲喂对照饮食或西式高脂高蔗糖饮食（Western diet）。实验中，按照每千克体重23.7mL的剂量，通过胃内插管（intra-gastric tubing）给予液态日粮，并于给药后120分钟采集血液样本。采样前，于股动脉与股静脉留置导管。采用连接1mL注射器的23G蝶翼针（23G butterfly needle），采集肝静脉、门静脉、肾静脉、脾静脉、肠系膜下静脉、耳外侧静脉、主动脉及冠状窦的血液样本；采用连接1mL注射器的27G针头，采集颈外静脉、左心室及右心室的血液样本。所有器官源性血液样本均于首次采样后的5~10分钟内完成采集。组织样本于终端手术后采集。