Transcription factor activating protein 2 beta (TFAP2B) mediates noradrenergic neuronal differentiation in neuroblastoma

Single-color gene expression profiles from IMR-32 neuroblastoma cell lines were generated using 44K oligonucleotide microarrays. To gain insights into the molecular processes occurring upon TFAP2B re-expression, we performed gene expression measurements in TFAP2B and GFP expressing transgenic IMR-32 neuroblastoma cell lines at d2 and d7 of transgene induction. Single-color gene expression profiles from IMR-32 neuroblastoma cell lines were generated using 44K oligonucleotide microarrays. Total RNA of TFAP2B- and GFP-expressing IMR-32 cells was isolated at day 2 and day 7 using Trizol.

本研究采用44K寡核苷酸微阵列（oligonucleotide microarrays），成功构建了IMR-32神经母细胞瘤细胞系的单色基因表达谱。为解析TFAP2B重新表达所介导的分子生物学过程，研究人员在转基因诱导的第2天与第7天，分别对表达TFAP2B与绿色荧光蛋白（GFP, Green Fluorescent Protein）的转基因IMR-32神经母细胞瘤细胞系开展了基因表达量检测。本研究同样采用44K寡核苷酸微阵列，完成了IMR-32神经母细胞瘤细胞系单色基因表达谱的构建。研究人员于转基因诱导的第2天与第7天，采用Trizol试剂提取了表达TFAP2B与GFP的IMR-32细胞的总RNA。