Nuclear Proteome

The nuclear proteome of mixed populations of Plasmodium falciparum extracted from infected erythrocytes was determined by GeLC-MS/MS and 2D-LC-MS/MS. A peptide exclusion procedure was used to increase the detection of low abundant proteins such as those involved in gene expression.从感染红细胞中提取的恶性疟原虫（Plasmodium falciparum）混合种群的核蛋白质组，通过凝胶洗脱液相色谱-串联质谱（GeLC-MS/MS）与二维液相色谱-串联质谱（2D-LC-MS/MS）进行了鉴定。为提升参与基因表达过程的低丰度蛋白质的检测效能，研究采用了肽段排除流程。