Retinal Leukocyte Infiltration Assay.

A) Mice were injected intraperitoneally with either PBS, Pam3CSK4 (25 µg per animal, in PBS), or dipeptide-CEP (400 µg per animal, in PBS) and the retinas were analyzed 8 hours later. Retinal infiltration by neutrophils (Gr1+ cells) or macrophages (F4/80+ cells) was assessed by immunostaining with the respective markers and quantitated with Axiovision, as described in Materials and Methods. Statistical analysis was performed using the Student t-test. Only statistically significant differences are indicated in the graph. B) Shown are representative images of the experiment in Figure 3A. Arrows indicate examples of macrophages or neutrophils in the corresponding images. CEP, Dipeptide-CEP; Pam3, Pam3CSK4.

A) 小鼠经腹腔注射给予磷酸盐缓冲液（PBS）、Pam3CSK4（每只动物25 μg，溶于PBS）或二肽-CEP（每只动物400 μg，溶于PBS），于给药8小时后分析视网膜组织。采用中性粒细胞（Gr1+细胞）与巨噬细胞（F4/80+细胞）的特异性标志物进行免疫染色，评估两类细胞在视网膜中的浸润情况，并按照材料与方法中的描述，通过Axiovision软件完成定量分析。统计学分析采用Student t检验，图表中仅标注具有统计学意义的组间差异。
B) 本部分展示了图3A对应实验的代表性图像，箭头指示对应图像中巨噬细胞或中性粒细胞的示例。
CEP：二肽-CEP；Pam3：Pam3CSK4。