Type I interferon protects against bone loss in periodontitis by mitigating an interleukin (IL)-17-neutrophil axis [RNA-Seq]

We assessed the transcriptomic profile at the late stage on day 10 at the end of the ligature-induced periodontitis model through RNA-seq. Overall design: We used the ligated gingival samples from the Ifnar1-/- mice and WT mice. The gingival tissues approximating the ligature were collected and RNA-seq were performed at the end of ligature model on day 10 post-ligature placement.

本研究采用RNA测序（RNA-seq）技术，对结扎诱导性牙周炎模型造模第10天（即模型终末期）的转录组表达谱开展了检测分析。实验整体设计：本研究以Ifnar1基因敲除（Ifnar1-/-）小鼠与野生型（WT）小鼠的结扎侧牙龈组织为实验材料。于结扎丝线放置后第10天，即结扎诱导性牙周炎模型造模结束时，收集紧邻结扎位点的牙龈组织，随后进行RNA测序实验。