Gene expression profile at single cell level of primary keloid fibroblasts treated with various radiotherapy modalities. Gene expression profile at single cell level of primary keloid fibroblasts treated with various radiotherapy modalities

Keloids are benign dermal tumors that arise from abnormal wound healing processes following skin lesions. Postoperative radiotherapy (PORT) is a clinically effective measure to reduce recurrence rates of keloid. We used single cell RNA sequencing (scRNA-seg) to analyze the different of primary keloid fibroblasts treated with various radiation modalities. Overall design: Primary keloid fibroblasts isolated from patients and analyzed using scRNA-seq.

瘢痕疙瘩（Keloids）是一类良性真皮肿瘤，由皮肤损伤后异常的伤口愈合过程引发。术后放疗（Postoperative radiotherapy, PORT）是临床中降低瘢痕疙瘩复发率的有效干预手段。本研究采用单细胞RNA测序（scRNA-seq），分析了经不同辐射方式处理的原代瘢痕疙瘩成纤维细胞的表达差异。实验设计：从患者体内分离原代瘢痕疙瘩成纤维细胞，并通过单细胞RNA测序开展相关分析。