Myocardial infarction time series single cell RNA-Seq

The outcome of cardiac repair post myocardial infarction is highly dependent on the balance between inflammation and fibrosis, which can lead to adverse ventricular remodeling and failure or early cardiac rupture. In order to profile the dynamic response of the interstitium to cardiac ischemic injury, we performed unbiased single cell RNA Sequencing (scRNAseq) on cardiac interstitial cells at homeostasis and 1, 3, 5, 7, 14, 28 days post-injury using transgenic mice on C57bl/6j background (B6), expressing ZsGreen under the control of the epicardial marker Wt1 (Wt1Cre;RosaZsgreenf/+). About 38,600 cells were captured using the 10xChromium technology. To gain insights on how cell composition and transcriptome can affect the predisposition to cardiac rupture, we compared the data on B6 background with 129S1/SvlmJ (129) mice sham and d3 post-MI (time point proceeding the rupture), capturing about 13,000 additional cells.

心肌梗死（myocardial infarction）后心脏修复的结局，高度依赖炎症与纤维化之间的平衡；该平衡失调可引发不良心室重构、心力衰竭，甚至早期心脏破裂。为解析心脏间质对心肌缺血性损伤的动态应答特征，本研究以C57BL/6J（B6）背景的转基因小鼠为模型——该小鼠在心外膜标志物Wt1的调控下表达ZsGreen，基因型为Wt1Cre;RosaZsgreenf/+——在稳态条件及损伤后1、3、5、7、14、28天，对心脏间质细胞开展了无偏倚的单细胞RNA测序（single cell RNA Sequencing，scRNAseq）。本研究通过10xChromium技术共捕获约38600个细胞。为探究细胞组成与转录组如何影响心脏破裂易感性，本研究将B6背景小鼠的测序数据与129S1/SvlmJ（129）小鼠的假手术组及心肌梗死后第3天（心脏破裂发生前的时间节点）的数据进行了对比，额外捕获约13000个细胞。