Sequencing of Hrip1-treated rice leaves

Hrip1 is an elicitor protein isolated from Alternaria tenuissima. It induces cell death and the expression of systematically acquired immunity. It was also identified to induce immunity against rice blast fungi. To investigate the immune machinery induced by Hrip1 treatment in rice seedlings, RNA-seq was performed on rice seedlings treated with 30 nm Hrip1 protein at the 3-leaf stage. The control plants were treated with Tris-HCl buffer. Triplicate samples from both experiments were taken at 6, 12, 24, and 48 hours after treatment. The control samples were given initial: CB and the Hrip1-treated were given initials: HT. each initial was followed by the time of sampling (6, 12, 24, and 48), and then the triplicate sample numbers followed (1, 2, and 3).

Hrip1是从细极链格孢（Alternaria tenuissima）中分离得到的激发子蛋白（elicitor protein）。该蛋白可诱导细胞死亡，并介导系统性获得免疫的表达，同时被证实能够诱导水稻产生抗稻瘟病菌的免疫反应。为探究Hrip1处理水稻幼苗所激活的免疫调控机制，研究人员对三叶期水稻幼苗施加30 nM的Hrip1蛋白进行处理，对照组则以Tris-HCl缓冲液处理，并对两组样本开展RNA-seq测序。两组实验均在处理后6、12、24及48小时四个时间点采集样本，且每个时间点设置3次生物学重复。对照组样本的命名前缀为CB，Hrip1处理组样本的命名前缀为HT；每个命名前缀后紧跟取样时间（6、12、24或48），随后再接重复样本的编号（1、2、3）。