Specific enrichment of hydrocarbonclastic bacteria from diesel-amended soil on biochar particles

Diesel oil contamination of soil causes highly-specific selection of conditionally-rare hydrocarbonoclasts from the soil microbial community. These microorganisms can be further enriched on biochar particles which are used to amend the contaminated soil. Extraction of soil bacterial communities after diesel contamination, followed by enrichment culture in the presence of biochar in medium with diesel oil as the sole carbon source, allows the formation of colonised biochar particles which can be used for it situ bioaugmentation.

土壤柴油污染可从土壤微生物群落中高度特异性地筛选出条件性稀有烃降解菌（hydrocarbonoclasts）。这类微生物可在用于修复污染土壤的生物炭（biochar）颗粒上进一步富集。在柴油污染后提取土壤细菌群落，随后在以柴油为唯一碳源的培养基中添加生物炭进行富集培养，可获得定植有该类微生物的生物炭颗粒，该颗粒可用于原位生物强化（bioaugmentation）。